Decoding directional genetic dependencies through orthogonal CRISPR/Cas screens
Michael Boettcher, Ruilin Tian, James A Blau, Evan Markegard, David Wu, Anne Biton, Noah Zaitlen, Frank P. McCormick, Martin Kampmann, Michael T. McManus
The paper introduces an orthogonal CRISPR/Cas screen to systematically activate one gene while knocking out another, enabling the identification and directionality of genetic interactions in human cancer cells.
bioRxiv · Mar 25, 2017 · PreprintMolecular Biology