Michael T. McManus

The paper identifies a co-dependency between CDK12 and the BAF chromatin-remodeling complex in TNBC, showing that their co-inhibition synergistically reduces cancer cell viability.

This study shows that using multiple guide RNAs in CRISPR screens can reduce cell numbers needed while maintaining screening effectiveness.

The paper investigates the role of Dux and MERVL in ZGA, totipotency, and cell toxicity using CRISPRa and a 2C-GFP reporter system, finding that MERVL activation contributes to expanded fate potential but not all totipotency features, while Dux-induced toxicity is independent of MERVL and involves Noxa.

The paper introduces poolVis and cellPool to improve scalability and decoding of protein barcode-based CRISPR screens through experimental and computational methods.

RainBar enables pooled live-cell imaging with single-cell resolution by using optical barcoding to track perturbations and analyze dynamic phenotypes.

This study shows that nucleolar maturation and rRNA synthesis are required to repress Dux and exit the 2-cell stage in embryos.

The paper evaluates druggable genes in mice and finds that many understudied ones have significant phenotypes similar to human diseases, suggesting therapeutic potential.

This paper introduces multi-level barcoding to address clonal heterogeneity in pooled genetic screens and demonstrates its ability to resolve cellular heterogeneity issues.

The paper shows that translation directly regulates chromatin state and transcription in ES cells, with reduced translation causing rapid chromatin changes.

The paper introduces an orthogonal CRISPR/Cas screen to systematically activate one gene while knocking out another, enabling the identification and directionality of genetic interactions in human cancer cells.