Michael Boettcher

The paper uses Perturb-seq to identify TCF7 as a key integrator of MAPK and Wnt signaling pathways through a feedback loop with EGR1.

The paper uses combinatorial CRISPR-Cas9 perturbations in brain organoids to uncover regulatory hierarchies of human-specific genes involved in cortical evolution.

This study uses targeted Perturb-seq to identify EGR1 and FOS as key regulators of the transcriptional RAF-MAPK response by constructing a directional interaction network.

The paper identifies X-chromosomal genes, including Klhl13, that drive sex differences in murine embryonic stem cells by affecting pluripotency and differentiation.

This paper introduces multi-level barcoding to address clonal heterogeneity in pooled genetic screens and demonstrates its ability to resolve cellular heterogeneity issues.

The paper introduces an orthogonal CRISPR/Cas screen to systematically activate one gene while knocking out another, enabling the identification and directionality of genetic interactions in human cancer cells.