Ruilin Tian

The paper identifies PQLC2 as a lysosomal pH regulator that affects tau homeostasis through a CRISPRi screen in iPSC-derived neurons.

The paper investigates the chromosomal contacts of poised enhancers during the transition from naive to primed human pluripotency, revealing their dynamics and functional significance.

CaRPOOL combines CRISPRi and CaMPARI2 to identify CCR7 as a modulator of osmomechanosensing through a PIEZO1-dependent pathway.

SPLiCR-seq identifies novel regulators of IRE1-XBP1 signaling under ER stress, revealing GADD34 as a key modulator with therapeutic potential in CAR-T cell immunotherapy.

This study identifies DDX39B as a global repressor of A-to-I RNA editing using CRISPR screens and shows its potential as a therapeutic target for anti-HDV therapy.

The paper develops JQ1-alkenyl oxindole conjugates that degrade BRD4 via the ubiquitin-proteasome system and recruits CRL4DCAF11, identifying HL435 as a potent antitumor lead.

The paper develops a CRISPRi-based screening platform to identify genes affecting neuronal excitability in human iPSC-derived neurons, revealing new links to neurodegenerative and neurodevelopmental disorders.

The paper represses 87 high-confidence ASD genes in a human cortical neurogenesis model, identifying 17 genes that disrupt developmental trajectory and reveal convergence on microtubule biology and RNA-binding proteins.

The paper introduces CrAAVe-seq, an AAV-based CRISPR screening platform for in vivo cell type-specific analysis, demonstrating its scalability and sensitivity in identifying neuron-essential genes.

The paper performs genome-wide CRISPRi/a screens in human neurons to identify gene functions related to oxidative stress and neurodegenerative diseases, finding that lysosomal failure links to ferroptosis.

The paper uses deep mutational scanning to explore how chemical perturbations affect ubiquitin's mutational tolerance, revealing sensitization of most positions except two.

The paper introduces an orthogonal CRISPR/Cas screen to systematically activate one gene while knocking out another, enabling the identification and directionality of genetic interactions in human cancer cells.