David Wu

High-resolution analysis of T cell subsets, clonality, and trajectories in psoriasis

This study analyzes T cell subsets, clonality, and trajectories in psoriasis using single-cell RNA and TCR sequencing, revealing tissue-specific and shared features of the T-cell repertoire.

Chromatin regulator Kdm6b is required for the establishment and maintenance of neural stem cells in mouse hippocampus

This study shows that Kdm6b is essential for establishing and maintaining neural stem cells in the mouse hippocampus, with loss of Kdm6b leading to defective neurogenesis and cognitive impairments.

Single-cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenic lineages

The paper uses single-cell and single nucleus sequencing to identify two distinct adult neurogenic lineages in the mouse V-SVZ, marked by specific gene expression patterns.

Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 Ribonucleoproteins

This paper describes a method to efficiently generate genetically modified primary human myeloid cells using CRISPR-Cas9 RNP nucleofection, demonstrating its utility in studying HIV-1 infection and myeloid cell biology.

Decoding directional genetic dependencies through orthogonal CRISPR/Cas screens

The paper introduces an orthogonal CRISPR/Cas screen to systematically activate one gene while knocking out another, enabling the identification and directionality of genetic interactions in human cancer cells.