F. Gielen

Thermostable in vitro transcription-translation for enzyme screening in microdroplets

This paper develops a thermostable in vitro transcription-translation system using Thermus thermophilus extracts for enzyme screening in microdroplets.

Investigating bacteria-phage interaction dynamics using droplet-based technology

This study uses droplet microfluidics and fluorescence microscopy to analyze bacterial-phage interactions in E. coli, revealing growth and lysis dynamics of phage-infected cells.

Label-free analysis of bacterial growth and lysis at the single-cell level using droplet microfluidics and object detection-oriented deep learning

This paper develops a label-free method using microfluidics and YOLOv4 deep learning to count and track single bacterial cells and study phage interactions.

High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform

This paper introduces a high-throughput droplet-based system for measuring enzyme kinetics with improved throughput and data quality compared to existing methods.

Cancer-associated fibroblast-derived ROR2 induces WNT/PCP activation and polarized migration in receiving gastric cancer cells.

The paper shows that cancer-associated fibroblasts transfer ROR2 to gastric cancer cells, activating WNT/PCP signaling and promoting migration.

Phenotyping single-cell motility in microfluidic confinement

The paper develops a microfluidic assay to study single-cell motility in response to environmental stimuli, revealing species-specific swimming behaviors and non-equilibrium dynamics.

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

The paper creates and tests chimeric BRC repeats to improve RAD51 binding, finding that shuffled modules can enhance binding affinity and function in human cells.