Epigenetic modification and characterization of the MGMT promoter region using CRISPRoff in glioblastoma cells
R. Weber, M. Weller, G. Reifenberger, F. Vasella
This study uses CRISPRoff to introduce site-specific CpG methylation in the MGMT promoter of glioblastoma cells, showing reduced MGMT expression and increased temozolomide sensitivity.
bioRxiv · Sep 21, 2023 · PreprintCancer Biology