Mechanism-guided mutagenesis of Rft1 to test its role as a dolichol-linked oligosaccharide scramblase in cells
George N. Chiduza, Ken‐taro Sakata, Hannah G. Wolfe, Faria Noor, Anant K. Menon
The paper uses computational models and mutagenesis to test if Rft1 is essential for M5-DLO scrambling in yeast, finding that scrambling is dispensable while M5-DLO binding is critical.
Protein Science · Jul 8, 2026Peer-reviewedBiochemistry