Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks
Baolei Yuan, Chongwei Bi, Yeteng Tian, Jincheng Wang, Yiqing Jin, Khaled N. Alsayegh, Muhammad Tehseen, Gang Yi, Xuan Zhou, Yanjiao Shao, Fernanda Vargas Romero, Wolfgang Fischle, Juan Carlos Izpisúa Belmonte, Samir M. Hamdan, Yanyi Huang, Mo Li
This study shows that modulating the MMEJ pathway components RPA and POLQ can reduce large deletions and enhance HDR after CRISPR-Cas9 editing.
BMC Biology · Apr 29, 2024Peer-reviewedMolecular Biology