Baolei Yuan

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks

This study shows that modulating the MMEJ pathway components RPA and POLQ can reduce large deletions and enhance HDR after CRISPR-Cas9 editing.

DMSO derives Trophectoderm and Clonal Blastoid from Single Human Pluripotent Stem Cell

This study shows that DMSO induces trophectoderm differentiation and blastoid formation from single human pluripotent stem cells without additional perturbations.

Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes

This paper introduces iMiGseq, a method for ultra-sensitive mtDNA sequencing in oocytes, revealing hidden heterogeneity and deleterious variants in human and mouse samples.

Long-read Individual-molecule Sequencing Reveals CRISPR-induced Genetic Heterogeneity in Human ESCs

The paper develops a method to quantify rare genetic variants in human cells using long-read sequencing and shows CRISPR-Cas9 induces nonrandom large deletions and insertions.