Juan Carlos Izpisúa Belmonte

The paper optimizes ATAC-see for high-throughput chromatin accessibility imaging in aging and reprogramming, revealing age-related chromatin changes and reprogramming-induced remodeling.

This study shows that unwinding RNA G-quadruplexes with FC3 induces and maintains totipotency in mouse and human cells, recapitulating 2-cell-stage features and enabling differentiation into embryonic and extraembryonic lineages.

This study shows that modulating the MMEJ pathway components RPA and POLQ can reduce large deletions and enhance HDR after CRISPR-Cas9 editing.

This study optimizes an AAV vector for efficient gene delivery to epidermal keratinocytes in vitro and in vivo by engineering capsid and promoter components.

The paper identifies regulatory network modules in human stem cells, showing epigenetic and genetic factors influence pluripotency state differences.

This study shows that DMSO induces trophectoderm differentiation and blastoid formation from single human pluripotent stem cells without additional perturbations.

The paper maps eQTLs in fetal-like pancreatic progenitor cells, identifying regulatory variants that influence diabetes risk by affecting gene expression and splicing in early development.

The paper develops universal epigenetic clocks that accurately estimate age across mammalian tissues using DNA methylation data from 185 species.

This study identifies convergence of aging and rejuvenation-related DNA methylation changes on PRC2 target genes in reprogrammed mouse skin.

This study uses interspecies neural blastocyst complementation to build and test functional rat-mouse neural circuits in rat-mouse brains, showing synaptic activity and partial behavioral rescue.

This study identifies causal genetic variants for cardiac traits by integrating spatiotemporal eQTL analysis with GWAS data, showing stage- and tissue-specific effects.

This paper introduces iMiGseq, a method for ultra-sensitive mtDNA sequencing in oocytes, revealing hidden heterogeneity and deleterious variants in human and mouse samples.

The paper investigates chromatin architecture changes during human cardiomyocyte differentiation and identifies HERV-H retrotransposons as key regulators of TAD boundaries and gene regulation.