Samir M. Hamdan

This study reveals the structural mechanism of aphidicolin inhibition of human DNA polymerases Pol δ and Pol ε, showing how it blocks DNA synthesis by altering the polymerase conformation.

This study presents cryo-EM structures of human DNA replication complexes to elucidate the structural basis of nick translation during Okazaki fragment maturation.

smDeepFLUOR uses deep learning to classify single-molecule fluorescence signals, distinguishing specific from nonspecific protein binding with 97% accuracy and capturing DNA synthesis kinetics.

This study presents a cryo-EM structure of the human CTF18-RFC complex bound to PCNA, revealing structural features important for PCNA loading and Pol ε-mediated leading strand synthesis.

The paper uses real-time imaging to study replisome rotation during DNA synthesis, finding that high-speed rotation supports continuous synthesis but reduces processivity and increases polymerase numbers.

This study shows PAF15 stabilizes PCNA on DNA, slows its sliding, and regulates its loading/unloading, impacting genome stability.

This study uses cryo-EM to visualize the dynamics of DNA unwinding by the SV40 Large Tumor Antigen helicase, revealing how ATP hydrolysis drives translocation and strand separation.

This paper revises the mechanism of hydroxyurea-induced cell cycle arrest in yeast, showing it is ROS-mediated rather than Mrc1-dependent, and introduces an improved alternative to HU called RNR-deg.

The paper uses Cryo-EM to determine the structure of Aquifex aeolicus lumazine synthase at 1.42 Angstrom resolution, revealing hydrogen atoms and confirming near-atomic resolution for protein complexes.

This study shows that modulating the MMEJ pathway components RPA and POLQ can reduce large deletions and enhance HDR after CRISPR-Cas9 editing.