Optimizing the Cell Painting assay for image-based profiling
Beth A. Cimini, Srinivas Niranj Chandrasekaran, Maria Kost‐Alimova, Lisa Miller, Amy B. Goodale, Briana Fritchman, Patrick J. Byrne, Sakshi Garg, Nasim Jamali, David J. Logan, John B. Concannon, Charles-Hugues Lardeau, Elizabeth Mouchet, Shantanu Singh, Hamdah Shafqat Abbasi, , Anne Elizabeth Carpenter
The paper updates the Cell Painting assay protocol to improve morphological profiling, finding that simplified steps and reduced stain concentrations maintain robust outputs.
Nature Protocols · Jun 21, 2023Peer-reviewedBioinformatics