Author

H. I. Alanen

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:protein folding & dynamics· 3 Research Focus:drug response / drug discovery· 2 Assay:proteomics· 2 Organism:E. coli· 1 Organism:human· 1 Assay:protein structure· 1 Approach:wet-lab / biochemical assay· 1

Frequent Co-Authors

L. Lehtiö· 3 shared A. Galera-Prat· 2 shared B. M. Paschal· 2 shared C. Vela-Rodriguez· 2 shared C. Yang· 2 shared M. M. Maksimainen· 2 shared A. Wagner· 1 shared F. Liu· 1 shared

Activity by Year

2021-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBiochemistry

L. Lehtiö

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Author profile →

4 papers

Recent·Most cited

Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor

C. Vela-Rodriguez, C. Yang, H. I. Alanen, R. Eki, T. A. Abbas, M. M. Maksimainen, T. Glumoff, R. Duman, A. Wagner, B. M. Paschal, L. Lehtiö

This study identifies the D2 domain of DTX3L as critical for forming oligomeric complexes that enable the DTX3L-PARP9 complex to read mono-ADP-ribosylated androgen receptor.

bioRxiv · Nov 29, 2023 · PreprintBiochemistry

Protein engineering approach to enhance activity assays of mono-ADP-ribosyltransferases through proximity

A. Galera-Prat, J. Alaviuhkola, H. I. Alanen, L. Lehtiö

The paper enhances PARP enzyme activity assays using a scaffold-based system, improving sensitivity and enabling more potent compound discovery.

bioRxiv · Apr 11, 2022Biochemistry

Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimer formation and enzymatic function

Y. Ashok, C. Vela-Rodriguez, C. Yang, H. I. Alanen, F. Liu, B. M. Paschal, L. Lehtiö

This study reconstitutes the DTX3L-PARP9 complex to identify determinants for high-affinity heterodimer formation and enzymatic function.

bioRxiv · Jun 14, 2021 · PreprintBiochemistry

A molecular toolbox for ADP-ribosyl binding proteins

S. T. Sowa, A. Galera-Prat, S. Wazir, H. I. Alanen, M. M. Maksimainen, L. Lehtio

The paper develops a molecular toolbox for studying ADP-ribosyl binding proteins, including a fusion tag and high-affinity binders, and identifies suramin as an inhibitor of SARS-CoV-2 nsp3 macrodomain.

bioRxiv · May 31, 2021Biochemistry