Author

L. Lehtiö

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 12

Signature Assays, Organisms & Methods

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Approach:experimental assay· 12 Research Focus:drug response / drug discovery· 7 Research Focus:protein folding & dynamics· 5 Organism:human· 4 Assay:protein structure· 2 Research Focus:protein structure prediction· 2 Approach:wet-lab / biochemical assay· 2 Organism:bacteria (other)· 1

Frequent Co-Authors

A. Galera-Prat· 5 shared M. M. Maksimainen· 5 shared Y. Ashok· 5 shared C. Vela-Rodriguez· 4 shared S. T. Sowa· 4 shared H. I. Alanen· 3 shared B. M. Paschal· 2 shared C. Yang· 2 shared

Activity by Year

2019-2023 · peak 5/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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5 shared papersBiochemistry

A. Galera-Prat

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12 papers

Recent·Most cited

Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor

C. Vela-Rodriguez, C. Yang, H. I. Alanen, R. Eki, T. A. Abbas, M. M. Maksimainen, T. Glumoff, R. Duman, A. Wagner, B. M. Paschal, L. Lehtiö

This study identifies the D2 domain of DTX3L as critical for forming oligomeric complexes that enable the DTX3L-PARP9 complex to read mono-ADP-ribosylated androgen receptor.

bioRxiv · Nov 29, 2023 · PreprintBiochemistry

High-throughput screening assay for PARP-HPF1 interaction inhibitors to affect DNA damage repair

S. S. Dhakar, A. Galera-Prat, L. Lehtiö

This study develops a FRET-based high-throughput screening assay to identify inhibitors of PARP1/2-HPF1 interaction, discovering two compounds with μM potency.

bioRxiv · Nov 14, 2023Biochemistry

Discovery of 2-amide-3-methylester thiophenes that target SARS-CoV-2 Mac1 and repress coronavirus replication, validating Mac1 as an anti-viral target

S. Wazir, T. A. O. Parviainen, M. M. Maksimainen, M. T. H. Duong, J. J. Pfannenstiel, D. Cluff, S. T. Sowa, A. Galera-Prat, D. V. Ferraris, A. Fehr, J. P. Heiskanen, L. Lehtiö

This paper identifies small molecule inhibitors of SARS-CoV-2 Mac1 that inhibit coronavirus replication, validating Mac1 as an anti-viral target.

bioRxiv · Aug 28, 2023 · PreprintBiochemistry

Discovery of DTX3L inhibitors through a homogeneous FRET-based assay that monitors formation and removal of poly-ubiquitin chains

C. Vela-Rodriguez, I. Scarpulla, Y. Ashok, L. Lehtiö

This paper develops a FRET-based assay to monitor DTX3L auto-ubiquitination and identifies low micromolar inhibitors, while also expanding the system to study deubiquitinases.

bioRxiv · Mar 13, 2023Biochemistry

The E3 ubiquitin ligase DTX3L and the deubiquitinase USP28 fine-tune DNA double strand repair through mutual regulation of their protein levels

D. Mennerich, Y. Ashok, C. Vela-Rodriguez, H. I. Hentilä, M. Rall-Scharpf, L. Wiesmüller, R. Prunskaite-Hyyryläinen, L. Lehtiö, T. Kietzmann

This paper identifies a regulatory feedback loop between DTX3L and USP28 that modulates DNA double-strand break repair by controlling protein levels and repair pathway efficiency.

bioRxiv · Jan 30, 2023Biochemistry

benzothiazole scaffold as versatile nicotinamide mimic allowing nanomolar inhibition of different PARP enzymes

S. Murthy, M. G. Nizi, M. M. Maksimainen, S. Massari, J. Alaviuhkola, B. E. Lippok, C. Vagaggini, S. T. Sowa, A. Galera-Prat, Y. Ashok, H. Venkannagari, R. Prunskaite-Hyyryläinen, E. Dreassi, B. Lüscher, P. Korn, , L. Lehtiö

The paper identifies a benzothiazole scaffold that inhibits multiple PARP enzymes with nanomolar potency, showing selectivity for mono-ARTs and poly-ARTs.

bioRxiv · Aug 29, 2022Biochemistry

Protein engineering approach to enhance activity assays of mono-ADP-ribosyltransferases through proximity

A. Galera-Prat, J. Alaviuhkola, H. I. Alanen, L. Lehtiö

The paper enhances PARP enzyme activity assays using a scaffold-based system, improving sensitivity and enabling more potent compound discovery.

bioRxiv · Apr 11, 2022Biochemistry

Discovery of compounds that inhibit SARS-CoV-2 Mac1-ADP-ribose binding by high-throughput screening

A. Roy, Y. M. Alhammad, P. McDonald, D. K. Johnson, J. Zhuo, S. Wazir, D. V. Ferraris, L. Lehtiö, A. Leung, A. R. Fehr

This study identifies 5 compounds that inhibit SARS-CoV-2 Mac1-ADP-ribose binding through high-throughput screening, with IC50 values under 100 µM.

bioRxiv · Mar 1, 2022Biochemistry

The zinc-binding motif in tankyrases is required for the structural integrity and proper function of the catalytic domain

S. T. Sowa, L. Lehtiö

This study investigates the role of the zinc-binding motif in tankyrases, showing it is critical for structural integrity and activity, with differential effects on TNKS1 and TNKS2.

bioRxiv · Oct 4, 2021 · PreprintBiochemistry

Analogs of TIQ-A as inhibitors of human mono-ADP-ribosylating PARPs

M. M. Maksimainen, S. Murthy, S. T. Sowa, A. Galera-Prat, E. Rolina, J. P. Heiskanen, L. Lehtiö

The paper develops TIQ-A analogs that selectively inhibit mono-ADP-ribosyltransferases PARP10 and PARP15 over poly-ADP-ribosyltransferases, achieving 10-20-fold selectivity while maintaining micromolar potency.

bioRxiv · Aug 30, 2021Biochemistry

Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimer formation and enzymatic function

Y. Ashok, C. Vela-Rodriguez, C. Yang, H. I. Alanen, F. Liu, B. M. Paschal, L. Lehtiö

This study reconstitutes the DTX3L-PARP9 complex to identify determinants for high-affinity heterodimer formation and enzymatic function.

bioRxiv · Jun 14, 2021 · PreprintBiochemistry

FMN-dependent oligomerization of putative lactate oxidase from Pediococcus acidilactici

Y. Ashok, M. M. Maksimainen, T. Kallio, P. Kilpeläinen, L. Lehtiö

The paper reports structural analysis of Pediococcus acidilactici lactate oxidase, revealing its FMN-dependent oligomerization and suggesting it may be a misannotated enzyme with unknown oxidation activity.

bioRxiv · Oct 2, 2019Biochemistry