Author

Y. Ashok

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:drug response / drug discovery· 3 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Research Focus:protein structure prediction· 2 Organism:bacteria (other)· 1 Research Focus:cancer & tumor microenvironment· 1 Research Focus:cell signaling & trafficking· 1

Frequent Co-Authors

L. Lehtiö· 5 shared C. Vela-Rodriguez· 3 shared M. M. Maksimainen· 2 shared R. Prunskaite-Hyyryläinen· 2 shared A. Galera-Prat· 1 shared A. Pulliainen· 1 shared A. Tiwari· 1 shared B. E. Lippok· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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5 shared papersBiochemistry

L. Lehtiö

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Discovery of DTX3L inhibitors through a homogeneous FRET-based assay that monitors formation and removal of poly-ubiquitin chains

C. Vela-Rodriguez, I. Scarpulla, Y. Ashok, L. Lehtiö

This paper develops a FRET-based assay to monitor DTX3L auto-ubiquitination and identifies low micromolar inhibitors, while also expanding the system to study deubiquitinases.

bioRxiv · Mar 13, 2023Biochemistry

The E3 ubiquitin ligase DTX3L and the deubiquitinase USP28 fine-tune DNA double strand repair through mutual regulation of their protein levels

D. Mennerich, Y. Ashok, C. Vela-Rodriguez, H. I. Hentilä, M. Rall-Scharpf, L. Wiesmüller, R. Prunskaite-Hyyryläinen, L. Lehtiö, T. Kietzmann

This paper identifies a regulatory feedback loop between DTX3L and USP28 that modulates DNA double-strand break repair by controlling protein levels and repair pathway efficiency.

bioRxiv · Jan 30, 2023Biochemistry

benzothiazole scaffold as versatile nicotinamide mimic allowing nanomolar inhibition of different PARP enzymes

S. Murthy, M. G. Nizi, M. M. Maksimainen, S. Massari, J. Alaviuhkola, B. E. Lippok, C. Vagaggini, S. T. Sowa, A. Galera-Prat, Y. Ashok, H. Venkannagari, R. Prunskaite-Hyyryläinen, E. Dreassi, B. Lüscher, P. Korn, , L. Lehtiö

The paper identifies a benzothiazole scaffold that inhibits multiple PARP enzymes with nanomolar potency, showing selectivity for mono-ARTs and poly-ARTs.

bioRxiv · Aug 29, 2022Biochemistry

Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimer formation and enzymatic function

Y. Ashok, C. Vela-Rodriguez, C. Yang, H. I. Alanen, F. Liu, B. M. Paschal, L. Lehtiö

This study reconstitutes the DTX3L-PARP9 complex to identify determinants for high-affinity heterodimer formation and enzymatic function.

bioRxiv · Jun 14, 2021 · PreprintBiochemistry

Discovery of compounds inhibiting the ADP-ribosyltransferase activity of pertussis toxin

Y. Ashok, M. Miettinen, D. K. H. de Oliveira, M. Z. Tamirat, K. Näreoja, A. Tiwari, M. O. Hottiger, M. S. Johnson, L. Lehtiï, A. Pulliainen

This study identifies two small molecules that inhibit the ADP-ribosyltransferase activity of pertussis toxin, showing potential for targeted antibiotic development against Bordetella pertussis.

bioRxiv · Nov 4, 2019Microbiology

FMN-dependent oligomerization of putative lactate oxidase from Pediococcus acidilactici

Y. Ashok, M. M. Maksimainen, T. Kallio, P. Kilpeläinen, L. Lehtiö

The paper reports structural analysis of Pediococcus acidilactici lactate oxidase, revealing its FMN-dependent oligomerization and suggesting it may be a misannotated enzyme with unknown oxidation activity.

bioRxiv · Oct 2, 2019Biochemistry