J. A. Escudero

The expression of integron arrays is shaped by the translation rate of cassettes

The paper shows that integron cassette expression depends on upstream translation rates, not distance to the Pc promoter.

IDENTIFICATION OF PROMOTER ACTIVITY IN GENE-LESS CASSETTES FROM VIBRIONACEAE SUPERINTEGRONS

The paper identifies promoter activity in gene-less cassettes from Vibrio superintegrons, showing they enhance downstream cassette expression and confer antibiotic resistance.

Chromosomal Integrons are Genetically and Functionally Isolated Units of Genomes

The paper deletes a chromosomal integron in Vibrio cholerae and shows it has no detectable effect on physiology, proving its genetic and functional isolation.

A rugged yet easily navigable fitness landscape of antibiotic resistance

The paper maps the fitness landscape of E. coli DHFR mutants under trimethoprim and finds that while rugged, high fitness peaks are accessible and have large basins of attraction.

Off-target integron activity leads to rapid plasmid compensatory evolution in response to antibiotic selection pressure

The paper shows integron activity can drive plasmid evolution by causing off-target inversions that improve plasmid stability and fitness under antibiotic pressure.

Integron activity accelerates the evolution of antibiotic resistance

This study shows that integron activity speeds up antibiotic resistance evolution in P. aeruginosa by enhancing cassette expression and removing redundant elements without harmful side effects.