Author

J. R. Holt

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 4 Ecology· 1 Immunology· 1 Zoology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Research Focus:cell signaling & trafficking· 4 Research Focus:cell type annotation· 4 Assay:imaging· 4 Organism:human· 3 Approach:statistical model· 3 Research Focus:host-pathogen & immune response· 2 Organism:mouse· 2

Frequent Co-Authors

M. M. Pathak· 4 shared E. L. Evans· 3 shared H. Abuwarda· 2 shared I. Parker· 2 shared J. L. Nourse· 2 shared A. Chang· 1 shared A. Mekhdjian· 1 shared A. Patapoutian· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 32 mutual co-author links

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4 shared papersBiophysics

M. M. Pathak

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Author profile →

7 papers

Recent·Most cited

Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology

G. A. Bertaccini, I. Casanellas, E. L. Evans, J. L. Nourse, G. D. Dickinson, G. Liu, S. Seal, A. T. Ly, J. R. Holt, T. D. Wijerathne, S. Yan, E. E. Hui, J. J. Lacroix, M. M. Panicker, S. Upadhyayula, , M. M. Pathak

This study uses HaloTag technology to visualize PIEZO1 localization and activity in hiPSC-derived cells and organoids, enabling mechanotransduction studies with high temporal resolution.

bioRxiv · Dec 22, 2023Biophysics

PIEZO1 regulates leader cell formation and cellular coordination during collective keratinocyte migration

J. Chen, J. R. Holt, E. L. Evans, J. S. Lowengrub, M. M. Pathak

This study investigates how PIEZO1 regulates leader cell formation and coordinated migration during keratinocyte wound healing, finding that PIEZO1 inhibits leader cell formation and disrupts coordinated directionality.

bioRxiv · Oct 13, 2022Biophysics

Protein Kinase C Delta Regulates Mononuclear Phagocytes and Hinders Response to Immunotherapy in Cancer

M. Chaib, J. R. Holt, L. M. Sipe, M. S. Bohm, S. C. Joseph, J. Yarbro, U. Tanveer, T. J. Hollingsworth, Q. Wei, P. Thomas, D. N. Hayes, L. Makowski

This study shows that PKCδ in mononuclear phagocytes suppresses antitumor immunity and response to immunotherapy by reprogramming macrophages and dendritic cells.

bioRxiv · Mar 31, 2022Immunology

Morphometric and Molecular Discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904)

S. Nibouche, L. Costet, R. F. Medina, J. R. Holt, J. Sadeyen, A.-S. Zoogones, P. Brown, R. L. Blackman

This study uses morphometric and molecular data to distinguish between two aphid species, finding that M. sorghi is the invasive genotype damaging sorghum in the US, Mexico, and the Caribbean since 2013.

bioRxiv · Oct 23, 2020Zoology

Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing

J. R. Holt, W.-Z. Zeng, E. L. Evans, S.-H. Woo, S. Ma, H. Abuwarda, M. Loud, A. Patapoutian, M. M. Pathak

This study shows that PIEZO1 channel localization dynamics regulate keratinocyte migration and wound healing speed in mice.

bioRxiv · Oct 18, 2020Biophysics

Myosin-II mediated traction forces evoke localized Piezo1 Ca2+ flickers

K. Ellefsen, J. R. Holt, A. Chang, J. L. Nourse, J. Arulmoli, A. Mekhdjian, H. Abuwarda, F. Tombola, L. A. Flanagan, A. R. Dunn, I. Parker, M. M. Pathak

The paper shows that Myosin-II mediated traction forces cause localized Piezo1 Ca2+ flickers in cells without external forces.

bioRxiv · May 13, 2019Biophysics

Landowner Functional Types to Characterize Response to Forest Insects

J. R. Holt, M. E. Borsuk, B. J. Butler, D. B. Kittredge, D. Laflower, M. G. MacLean, M. Markowski-Lindsay, D. Orwig, J. R. Thompson

The paper characterizes landowner functional types based on responses to forest insects and pathogens and models their distribution across New England.

bioRxiv · Apr 2, 2019Ecology