Jacob Ratcliffe

Structural and biochemical characterization of LIG1 during mutagenic nick sealing of oxidatively damaged ends at the final step of DNA repair

This study characterizes LIG1's structural and biochemical interactions with oxidatively damaged DNA during the final steps of base excision repair.

Characterization of nick binding and sealing by LIG1 Huntington's disease-asssociated K845N variant at biochemical, structural, and single-molecule levels

This study examines how the K845N mutation in LIG1 affects nick binding and sealing using biochemical, structural, and single-molecule approaches, revealing reduced ligation efficiency and altered binding dynamics.

Mutagenic ligation of polβ mismatch insertion products during 8-oxoG bypass by LIG1 and LIG3α at the downstream steps of base excision repair pathway

This study investigates how LIG1 and LIG3α handle nick sealing after polβ inserts dATP or dCTP during 8-oxoG bypass, showing ribonucleotide insertions disrupt repair coordination.