CRISPR-PTM and CRISPR-VEIS: Multiplexed platforms for quantitative functional analysis of endogenous phosphosites
Simon Willaume, Jan Benada, Karen Akopyan, Valdemaras Petrosius, Jayashree Vijay Thatte, Thomas C. R. Miller, Arne Lindqvist, Claus Storgaard Sørensen
This paper introduces CRISPR-PTM and CRISPR-VEIS to quantitatively analyze endogenous phosphosite effects on cellular fitness and signaling, showing Y19 as a novel inhibitory site in the WEE1-CDK1 pathway.
bioRxiv · May 9, 2026 · PreprintCell Biology