An efficient eukaryotic cell-free expression and correlative cryo-electron tomography platform
Vikas A. Tillu, Dominic J. B. Hunter, Kai‐En Chen, Jessica Smith, Olivia Nassar, James A. Rae, Emma Sierecki, Boštjan Kobe, Brett M. Collins, Yann Gambin, Robert G. Parton, Nicholas Ariotti
The paper develops a method to express and visualize eukaryotic proteins using cell-free systems and cryo-electron tomography, demonstrating its application to study ASC protein filaments and their interaction with ribosomes.
bioRxiv · Oct 18, 2025 · PreprintBiochemistry