Boštjan Kobe

This study determines the crystal structure of AbTir, revealing how its coiled-coil domain inhibits NAD+ nucleosidase activity by disrupting symmetry and preventing NAD+ binding site formation.

This study shows that 3'cADPR-induced SIR2 filament formation activates ThsA effectors in type I Thoeris systems across bacterial species.

The paper determines cryo-EM structures of a plant TNL NLR, revealing its activation mechanism and NAD+ recognition, which informs engineering disease-resistant crops.

The paper identifies a natural genetic variant in the RPS4/RRS1 NLR pair that confers thermostable resistance to Ralstonia pseudosolanacearum in Arabidopsis thaliana.

The paper develops a method to express and visualize eukaryotic proteins using cell-free systems and cryo-electron tomography, demonstrating its application to study ASC protein filaments and their interaction with ribosomes.

This study analyzes the TIRome of Arabidopsis to design artificial TIR proteins and identify functional polymorphisms that influence cell death and metabolite production.

The paper introduces GRASP, a method for ancestral sequence reconstruction that handles large datasets and indels, demonstrating its ability to generate biologically active enzyme ancestors.

This study identifies VMN, a metabolite of the pesticide vacor, as a potent SARM1 activator, showing that SARM1 removal rescues vacor-induced neuron and axon death.

The paper identifies nicotinic acid mononucleotide (NaMN) as an allosteric inhibitor of SARM1 that promotes axonal protection by blocking NMN binding and stabilizing the inactive SARM1 conformation.

This paper develops an evolutionary model of protein secondary structure to reveal biological relationships not evident in amino acid-based analysis.

This study investigates how pathological mutations affect the prion-like polymerization of MyD88, showing that these mutations block polymerization and alter oligomer stability.