Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore
John R. Tyson, Phillip L. James, David Stoddart, Natalie Sparks, Arthur Wickenhagen, Grant Hall, Ji Hyun Choi, Hope R. Lapointe, Kimia Kamelian, Andrew D. Smith, Natalie Prystajecky, Ian Goodfellow, Sam J. Wilson, Richard Harrigan, Terrance P. Snutch, , Joshua Quick
V3 primer pools with 22 extra alternates improve coverage; revised library preps raise double-barcode demultiplexing from 42.15% to 74.5%, cut misassignment 35x and bring reagent cost near £10 per sample.
bioRxiv · Sep 4, 2020 · PreprintGenomics✦ In-depth digest