Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryos
Honglin Chen, Wenxin Hu, Valeria Impicciche, Gurjeet Jagjeet Singh, Joshua King, Carolyn Shembrey, Priyank Rawat, Joshua M. L. Casan, Srdjan Boskovic, Scott Paterson, Wei Dong Zhao, Sharon R. Lewin, Ricky W. Johnstone, Benjamin M. Hogan, Stephin J. Vervoort, , Mohamed Fareh
This study shows that target RNA abundance controls collateral activity of RfxCas13d in human cells and zebrafish embryos, with high abundance causing widespread degradation and toxicity.
bioRxiv · Apr 8, 2026 · PreprintMolecular Biology