Substrate scope and catalytic mechanism of α, β-epoxyketone synthase EpnF illuminated by in-situ esterase-mediated deprotection
Callum J Bullock, Marlene L. Rothe, Joshua W. Cartwright, Munro Passmore, Alex J. Mullins, Fabrizio Alberti, Józef Romuald Lewandowski, Lona M. Alkhalaf, Gregory L. Challis
This paper develops an esterase-mediated strategy to generate unstable substrates for EpnF, revealing its broad substrate tolerance and catalytic mechanism for epoxyketone synthesis.
ACS Catalysis · Jun 25, 2026Peer-reviewedBiochemistry