Author

Marlene L. Rothe

University of WarwickORCID 0000-0002-8261-7500Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 1 Approach:benchmark study· 1 Research Focus:benchmarking· 1 Research Focus:database / resource· 1 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Organism:fungi (other)· 1

Frequent Co-Authors

Fabrizio AlbertiiD· 2 shared Gregory L. ChallisiD· 2 shared Lona M. AlkhalafiD· 2 shared Alex J. MullinsiD· 1 shared Barbara R. TerlouwiD· 1 shared Callum J Bullock· 1 shared Christopher D. FageiD· 1 shared Chuan HuangiD· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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2 shared papersBiochemistry

Fabrizio AlbertiORCID

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2 papers

Recent·Most cited

Substrate scope and catalytic mechanism of α, β-epoxyketone synthase EpnF illuminated by in-situ esterase-mediated deprotection

Callum J Bullock, Marlene L. Rothe, Joshua W. Cartwright, Munro Passmore, Alex J. Mullins, Fabrizio Alberti, Józef Romuald Lewandowski, Lona M. Alkhalaf, Gregory L. Challis

This paper develops an esterase-mediated strategy to generate unstable substrates for EpnF, revealing its broad substrate tolerance and catalytic mechanism for epoxyketone synthesis.

ACS Catalysis · Jun 25, 2026Peer-reviewedBiochemistry

PARAS: high-accuracy machine-learning of substrate specificities in nonribosomal peptide synthetases

Barbara R. Terlouw, Chuan Huang, David Meijer, José D D Cediel-Becerra, Ruolin He, Marlene L. Rothe, Matthew Jenner, Shanshan Zhou, Yu Zhang, Christopher D. Fage, Yuta Tsunematsu, Gilles P. van Wezel, Serina L. Robinson, Fabrizio Alberti, Lona M. Alkhalaf, , Marnix H. Medema

Random forest predictors PARAS and PARASECT, trained on 3,653 curated adenylation domains, outperform earlier NRPS substrate predictors and correctly assigned L-Trp to an unusual tryptopeptin synthetase domain.

JACS Au · Apr 10, 2026Peer-reviewedBioinformatics✦ In-depth digest