Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro
K. Shintomi, Y. Masahara-Negishi, M. Shima, S. Tane, T. Hirano
This study develops a method to express and purify recombinant cyclin B-Cdk1 and demonstrates its ability to perform multi-site phosphorylation in vitro, including on the condensin I complex.
bioRxiv · Sep 14, 2023 · PreprintCell Biology