T. Hirano

Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro

This study develops a method to express and purify recombinant cyclin B-Cdk1 and demonstrates its ability to perform multi-site phosphorylation in vitro, including on the condensin I complex.

Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit

This study shows that the N-terminal tail of CAP-H regulates condensin I loading in a cell cycle-specific manner through phosphorylation and deletion effects in Xenopus extracts.

Elasticity control of entangled chromosomes: crosstalk between condensin complexes and nucleosomes

The paper constructs a theoretical model to explain the formation of a unique bean-shaped structure formed by mutant condensin complexes and nucleosomes in Xenopus egg extracts.

Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays

This study investigates the role of condensin II subunits in chromosome assembly using Xenopus egg extracts, revealing distinct functional roles of CAP-D3 and CAP-G2.

The SYP123-VAMP727 SNARE complex is involved in the delivery of inner cell wall components to the root hair shank in Arabidopsis

The paper identifies SYP123 and VAMP727 as components of a SNARE complex involved in delivering inner cell wall materials to the root hair shank in Arabidopsis.

Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex

This study reports the crystal structure of the human condensin I subcomplex hCAP-G and hCAP-H, revealing their interaction and DNA binding capabilities essential for mitotic chromosome assembly.

Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms

This study shows Ki-67 and condensins have independent yet cooperative roles in maintaining mitotic chromosome structure through distinct mechanisms.