ImmunologybioRxivPreprint
Fixation Before Dissociation Using a Deep Eutectic Solvent Preserves In Vivo States and Phospho-Signaling in Single-Cell Sequencing
S. D. Fortmann, B. F. Frey, V. S. Hanumanthu…·R. S. Welner
This study introduces a deep eutectic solvent fixation method that preserves in vivo cellular states and phospho-signaling for scRNA-seq, enabling the identification of hyperactivated Th17 T cells.