Synthetic BiologybioRxivPreprint
SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in Deinococcus radiodurans
Stephanie L. Brumwell, Katherine D. Van Belois, Daniel P. Nucifora, Bogumil Jacek Karas
The paper introduces SLICER, a method for seamless genome modification in D. radiodurans by recycling selectable markers to delete multiple R-M system genes.
DOI 10.1101/2022.10.15.512367