Author

Daniel P. Nucifora

Western UniversityORCID 0000-0002-4443-9184Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:Arabidopsis· 1 Organism:bacteria (other)· 1 Organism:Drosophila· 1 Assay:whole-genome sequencing· 1

Frequent Co-Authors

Bogumil Jacek KarasiD· 3 shared Daniel J. GiguereiD· 1 shared Katherine D. Van Belois· 1 shared Nidhi D. Mehta· 1 shared Stephanie L. BrumwelliD· 1 shared Tanveer S. Saini· 1 shared

Activity by Year

2022-2026 · peak 1/yr

Co-Author Collaboration Network

6 top collaborators · 7 mutual co-author links

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3 shared papersSynthetic Biology

Bogumil Jacek KarasORCID

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3 papers

Recent·Most cited

Synthetic BiologyACS Synthetic BiologyPeer-reviewedApr 6, 2026

Direct Genome Transfer from Acholeplasma laidlawii to Yeast

Daniel P. Nucifora, Tanveer S. Saini, Bogumil Jacek Karas

The paper demonstrates cloning the genome of Acholeplasma laidlawii into yeast by removing a toxic gene and using cell fusion, achieving successful genomic integration in 13 out of 20 colonies.

3 citationstop 1%

bacteria (other)experimental assay

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Synthetic BiologyACS Synthetic BiologyPeer-reviewedDec 19, 2023

An expanded genetic toolbox to accelerate the creation of Acholeplasma laidlawii driven by synthetic genomes

Daniel P. Nucifora, Nidhi D. Mehta, Daniel J. Giguere, Bogumil Jacek Karas

This paper develops genetic tools for Acholeplasma laidlawii to enable synthetic genome-based strain engineering.

5 citations

Arabidopsiswhole-genome sequencing

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Synthetic BiologybioRxivPreprintOct 15, 2022

SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in Deinococcus radiodurans

Stephanie L. Brumwell, Katherine D. Van Belois, Daniel P. Nucifora, Bogumil Jacek Karas

The paper introduces SLICER, a method for seamless genome modification in D. radiodurans by recycling selectable markers to delete multiple R-M system genes.

DOI 10.1101/2022.10.15.512367

Drosophilaexperimental assay

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