Stephanie L. Brumwell

A scalable transposon mutagenesis system for non-model bacteria

This paper develops a scalable transposon mutagenesis system for non-model bacteria, identifying functional configurations and applying them to study gene function in Comamonas testosteroni.

A genetic platform for a biocementation bacterium

This paper develops a genetic toolkit for Sporosarcina pasteurii, enabling genome modification and uncovering genes involved in biocementation.

Active learning guides automated discovery of DNA delivery via electroporation for non-model microbes

This paper develops an automated platform using active learning to discover electroporation protocols for non-model microbes, achieving higher transformation efficiency for industrial chassis.

Functional genomics in a microbe that degrades and metabolizes PET plastic

This study develops genetic tools for Piscinibacter sakaiensis to enable functional genomics and engineering for PET degradation.

A reference set of functional plasmids for Vibrio natriegens

The paper identifies functional plasmids for Vibrio natriegens using the POSSUM toolkit, showing compatibility of multiple replicons in wild-type and engineered strains.

Engineering Conjugative Plasmids for Inducible Horizontal DNA Transfer

This study develops inducible conjugative plasmids for controlled horizontal gene transfer to combat antimicrobial resistance, showing increased conjugation efficiency upon induction.

Design and construction towards a pan-microbial toolkit

The paper designs and constructs a pan-microbial genetic toolkit using high-throughput methods to identify functional genetic parts in non-model microbes.

A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria

The paper introduces a scalable framework to identify functional origins of replication in non-model bacteria using a combinatorial plasmid library and next-generation sequencing.

SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in Deinococcus radiodurans

The paper introduces SLICER, a method for seamless genome modification in D. radiodurans by recycling selectable markers to delete multiple R-M system genes.

Conjugation-based genome engineering in Deinococcus radiodurans

This paper develops a conjugation-based system for genome engineering in D. radiodurans, enabling stable plasmid introduction and gene deletion protocols.

Rapid method for generating designer algal mitochondrial genomes

The paper develops a method to generate designer algal mitochondrial genomes using yeast and E. coli hosts for efficient DNA cloning and testing.

Designer Sinorhizobium meliloti strains and multi-functional vectors for direct inter-kingdom transfer of high G+C content DNA

The paper develops S. meliloti strains and vectors to transfer high G+C content DNA to multiple hosts.