Laura Day

Massively parallel identification of causal variants underlying gene expression differences in a yeast cross

The paper identifies 451 causal regulatory variants in yeast promoters using a massively parallel reporter assay and finds they show epistasis, conservation, and enrichment in transcription factor binding sites.

Rare variants contribute disproportionately to quantitative trait variation in yeast

This study shows rare DNA variants have a larger impact on quantitative traits in yeast compared to common variants, due to their greater effect sizes and recent evolutionary origins.

Genetics of trans-regulatory variation in gene expression

This study identifies eQTLs in yeast to show that most gene expression variation is driven by distant, trans-acting eQTLs clustered at hotspots.

Highly parallel genome variant engineering with CRISPR/Cas9 in eukaryotic cells

The paper uses CRISPR/Cas9 in yeast to engineer and screen variants, finding most premature termination codons are deleterious except near the C-terminal end.

CRISPR-directed mitotic recombination enables genetic mapping without crosses

This study uses CRISPR to create targeted recombination events in yeast, enabling fine-mapping of trait variation to a single causal polymorphism.