Meru J. Sadhu

Ktd1 is a phospho-regulated member of the Dup240 family that mediates defence against killer toxin K28

This study identifies Ktd1 as a phospho-regulated member of the Dup240 family essential for defense against the K28 killer toxin in yeast.

Ancestral Quadruplication of Protein Kinase R in Tarsiers Hints Towards Novel Primate-Virus Innovations

This study identifies a unique PKR gene quadruplication in tarsiers, which may represent an evolutionary strategy to counteract viral inhibition.

High-throughput Eukaryotic Screening of Putative Anti-CRISPR Proteins against SpyCas9 and SauCas9

The paper screens hundreds of anti-CRISPR homologs to inhibit SpyCas9 and SauCas9, revealing evolutionary patterns and discovering three novel Acr families.

Combinatorial mosaics dissect the residue level basis of ACE2 binding divergence between SARS-CoV-2 and RaTG13

The paper constructs combinatorial RBD mosaics of SARS-CoV-2 and RaTG13 to identify residue-level differences affecting ACE2 binding affinity.

Systematic screen of PKR reveals genetic variants that broadly evade divergent viral pseudosubstrate inhibitors

This study screens genetic variants of PKR to identify those that resist diverse viral pseudosubstrate inhibitors, finding shared resistance mechanisms across different inhibitors.

Improved vectors for retron-mediated CRISPR-Cas9 genome editing in Saccharomyces cerevisiae

The paper develops plasmids for CRISPR-Cas9 genome editing in yeast using retron-mediated amplification of repair templates, achieving high editing efficiencies.

Highly replicated experiments studying complex genotypes using nested DNA barcodes.

The paper introduces a barcode-based method to track combinatorial genotypes in pooled experiments, enabling high-throughput genetic screening and analysis of complex genotype-phenotype relationships.

Killer Toxin K28 resistance in yeast relies on COG complex mediated trafficking of the defence factor Ktd1

This study identifies the COG complex as essential for Ktd1 trafficking, which mediates resistance to the A/B toxin K28 in yeast.

Comprehensive deletion scan of anti-CRISPR AcrIIA4 reveals essential and dispensable domains for Cas9 inhibition

The paper develops a high-throughput method to identify essential and dispensable domains in AcrIIA4 by generating and testing precise deletions, revealing that PAM binding inhibition is key while catalytic domain interaction is dispensable.

Systematic genetic characterization of the human PKR kinase domain highlights its functional malleability to escape a poxvirus substrate mimic

This study identifies K3-resistant variants in the PKR kinase domain and shows their enrichment under positive selection and resilience to viral adaptation.

Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3

The paper systematically explores genetic variants in human PKR and vaccinia virus K3 to understand their evolutionary dynamics and functional interactions.

Long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity

The paper uses long-read sequencing to identify pangenomic genes contributing to yeast phenotypic variation by resolving QTLs that are missed by short-read methods.

Genome-wide base editor screen identifies regulators of protein abundance in yeast

The paper uses a CRISPR base editor screen in yeast to identify regulators of protein abundance, revealing both specific and broad regulatory relationships.

KTD1 is a yeast defense factor against K28 killer toxin

The paper identifies KTD1 as a novel defense factor in Saccharomyces cerevisiae that confers resistance to the K28 killer toxin.

Rare variants contribute disproportionately to quantitative trait variation in yeast

This study shows rare DNA variants have a larger impact on quantitative traits in yeast compared to common variants, due to their greater effect sizes and recent evolutionary origins.

Analysis of the genetic basis of height in large Jewish nuclear families

This study identifies genetic loci contributing to height variation in a Jewish population cohort, explaining 6% of the variance through rare variants.

Highly parallel genome variant engineering with CRISPR/Cas9 in eukaryotic cells

The paper uses CRISPR/Cas9 in yeast to engineer and screen variants, finding most premature termination codons are deleterious except near the C-terminal end.

Accounting for genetic interactions improves modeling of individual quantitative trait phenotypes in yeast

The paper shows that accounting for genetic interactions improves modeling of quantitative trait phenotypes in yeast by considering epistatic networks and allele frequencies.