GenomicsGenome ResearchPeer-reviewed
Non-destructive enzymatic deamination enables single molecule long read sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single base resolution.
Zhiyi Sun, Romualdas Vaisvila, Laura-Madison Hussong…·Laurence M. Ettwiller
This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.
97 citations