Bo Yan

This study introduces RIMS-seq2, a method to simultaneously sequence the genome and methylome with minimal changes to DNA sequencing protocols, showing accurate variant calling and methylation mapping.

The paper discovers novel DNA cytosine deaminases and develops a nondestructive single-enzyme methylation sequencing method for high-coverage methylome mapping of low-input DNA.

Methyl-SNP-seq captures both methylation and sequence information from single DNA molecules, enabling analysis of allele-specific methylation and methyltransferase specificity.

ReCappable-seq identifies TSS for all RNA polymerases at single-nucleotide resolution, revealing novel TSS in nuclear and mitochondrial genomes and expanding understanding of non-Pol-II transcription.

RIMS-seq identifies methylase specificities and sequences bacterial genomes using a simple protocol, revealing new methylase targets in bacteria and synthetic communities.

This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.

The paper introduces a computational framework, SeqATU, to predict alternative transcription units in bacterial genomes using RNA-Seq data, achieving high precision and recall in E. coli.

SMRT-Cappable-seq reveals extended operons and read-through termination sites in E. coli, showing most bacterial genes are part of multiple operon variants.