Romualdas Vaisvila

Discovery of novel DNA cytosine deaminase activities enables a nondestructive single-enzyme methylation sequencing method for base resolution high-coverage methylome mapping of cell-free and ultra-low input DNA

The paper discovers novel DNA cytosine deaminases and develops a nondestructive single-enzyme methylation sequencing method for high-coverage methylome mapping of low-input DNA.

Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution.

Methyl-SNP-seq captures both methylation and sequence information from single DNA molecules, enabling analysis of allele-specific methylation and methyltransferase specificity.

A Genome-Phenome Association study in native microbiomes identifies a mechanism for cytosine modification in DNA and RNA

This study introduces MetaGPA, a framework linking metagenomic genetic data to functional phenotypes, and identifies a novel cytosine modification mechanism in DNA and RNA.

EM-seq: Detection of DNA Methylation at Single Base Resolution from Picograms of DNA

EM-seq detects DNA methylation at single base resolution using less DNA than bisulfite sequencing, showing improved accuracy and reliability.

Non-destructive enzymatic deamination enables single molecule long read sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single base resolution.

This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.