BiochemistrybioRxivPreprint
Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein
Siu‐Hong Chan, Louise Tirole, Daniel W. Kneller…·G. Brett Robb
This paper describes an RNA capping enzyme-T7 RNA polymerase fusion protein that efficiently generates capped synthetic mRNA in a one-step process, achieving up to 95% Cap-1 incorporation.