This study identifies and characterizes PC04 and PC05, two cap-specific N6-adenosine methyltransferases, and demonstrates their use in synthesizing m6A Cap-1 mRNA in vitro.

bioRxiv · Oct 23, 2026 · PreprintBiochemistry

Structural Insights into the Assembly and Regulation of 2'-O RNA Methylation by SARS-CoV-2 nsp16/nsp10

Anurag Misra, R. Rahisuddin, Manish Parihar, Shailee Arya, Thiruselvam Viswanathan, Nathaniel D. Jackson, Shan Qi, Siu‐Hong Chan, Reuben S. Harris, Luis Martínez‐Sobrido, Yogesh K. Gupta

This study presents a 2.4 Å crystal structure of the SARS-CoV-2 nsp16/nsp10 complex bound to Cap-1 RNA, revealing structural insights into its assembly and methylation mechanism.

Structure · Apr 11, 2025Peer-reviewedMicrobiology

In vitro characterization of the yeast DcpS, a scavenger mRNA decapping enzyme

Madalee G. Wulf, John Buswell, Siu‐Hong Chan, Nan Dai, Katherine Marks, Evan R. Martin, George Tzertzinis, Joseph M. Whipple, Ivan Reis Correa Junior, Ira Schildkraut

This study characterizes the yeast DcpS enzyme's ability to decap long RNA transcripts and validates its use for enriching capped RNA through a tagging strategy.

Scientific Reports · Jun 13, 2019Peer-reviewedMolecular Biology