M. D. Lew

Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay

This study uses SMOLM to analyze A{beta}42 fibril architecture and dynamics, finding that order and heterogeneity correlate with growth and decay.

Resolving the nanoscale structure of β-sheet assemblies using single-molecule orientation-localization microscopy

This study uses single-molecule orientation-localization microscopy to analyze the structure and polymorphism of engineered and pathological cross-beta fibrils.

POLCAM: Instant molecular orientation microscopy for the life sciences

POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.

Single fluorogen imaging reveals distinct environmental and structural features of biomolecular condensates

This study uses single-fluorogen imaging to reveal that biomolecular condensates have hydrophobic internal environments and spatially inhomogeneous molecular organization.

Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope

The paper introduces a raMVR microscope that achieves 6D super-resolution imaging of single molecules with high precision in 3D position and orientation.

Resolving the 3D rotational and translational dynamics of single molecules using radially and azimuthally polarized fluorescence

This paper introduces a radially and azimuthally polarized microscope to measure single-molecule orientation and dynamics with high precision, revealing cholesterol-induced changes in membrane behavior.

Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble using a Polarized Vortex Point Spread Function

This paper introduces a polarized vortex PSF to simultaneously measure 3D orientation and anisotropic wobble of single molecules from a single image, achieving high precision in localization, orientation, and wobble.

Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

This paper introduces a method to measure the accuracy of single-molecule localization microscopes directly on experimental data without ground-truth knowledge.

Single-molecule 3D orientation imaging reveals nanoscale compositional heterogeneity in lipid membranes

The paper introduces SMOLM, a single-molecule orientation localization microscopy technique, to measure orientation and spatial organization of lipophilic probes in lipid membranes, revealing compositional heterogeneity.

Single-molecule orientation localization microscopy for resolving structural heterogeneities between amyloid fibrils

The paper introduces a new performance metric, VUB, to efficiently evaluate orientation measurement performance of PSFs and applies it to study structural heterogeneities in amyloid fibrils using single-molecule orientation localization microscopy.