Author
Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.
7 papers
Recent·Most cited
B. Zhang, S. Daly, C. Zhu, M. O. Lenz, L. E. Weiss, L.-M. Needham, R. Peters, S. Lee, K. O'Holleran
This paper introduces vortex light field microscopy, achieving 3D spectral single-molecule imaging with 25 nm spatial and 3 nm spectral precision.
bioRxiv · Jul 18, 2024Biophysics
L.-M. Needham, C. Saavedra, J. K. Rasch, D. Sole-Barber, B. S. Schweitzer, A. J. Fairhall, C. H. Vollbrecht, B. Mehlenbacher, Z. Zhang, L. Tenbrake, H. Pfeifer, E. R. Chapman, R. H. Goldsmith
This paper introduces a label-free technique to detect individual solution-phase molecules using microcavities, achieving high signal-to-noise ratios and resolving molecular radius and isomer mixtures.
bioRxiv · Mar 24, 2023Biophysics
E. Bruggeman, O. Zhang, L.-M. Needham, M. Koerbel, S. Daly, M. Cheetham, R. Peters, T. Wu, A. S. Klymchenko, S. J. Davis, E. K. Paluch, D. Klenerman, M. D. Lew, K. O'Holleran, S. F. Lee
POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.
bioRxiv · Feb 7, 2023Biophysics
A. Vilalta, Y. Zhou, J. Sevalle, J. K. Griffin, K. Sato, D. H. Allendorf, S. De, M. Puigdellivol, A. Bruzas, M. A. Burguillos, R. B. Dodd, F. Chen, Y. Zhang, P. Flagmeier, L.-M. Needham, , G. C. Brown
The paper shows wild-type sTREM2 inhibits Aβ aggregation and neurotoxicity, while the R47H variant promotes Aβ aggregation and neurotoxicity.
bioRxiv · Dec 3, 2020Biochemistry
S. Basu, O. Shukron, D. Hall, P. Parruto, A. Ponjavic, D. Shah, W. Boucher, D. Lando, W. Zhang, N. Reynolds, L. H. Sober, A. Jartseva, R. Ragheb, X. Ma, J. Cramard, , E. D. Laue
This study uses live-cell 3D single-molecule tracking to show that the NuRD complex decompacts chromatin and alters enhancer dynamics by increasing their movement and reducing time in a fast-diffusing state.
bioRxiv · Apr 3, 2020 · PreprintCell Biology
A. Spark, A. Kitching, D. Esteban-Ferrer, A. Handa, A. R. Carr, L.-M. Needham, A. Ponjavic, M. Da Cunha Santos, J. McColl, C. Leterrier, S. J. Davis, R. Henriques, S. F. Lee
vLUME is a VR software for visualizing 3D SMLM data, enabling segmentation and quantification of fluorescence puncta with high fidelity.
bioRxiv · Jan 20, 2020Biophysics
L.-M. Needham, J. Weber, J. A. Varela, J. W. B. Fyfe, D. T. Do, C. K. Xu, B. Keenlyside, R. Cliffe, D. Klenerman, C. M. Dobson, C. A. Hunter, S. E. Bohndiek, T. N. Snaddon, S. F. Lee
This paper introduces ThX, a new fluorescent probe for detecting amyloid aggregates, showing improved brightness and binding affinity over ThT.
bioRxiv · Jul 24, 2019Biophysics