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10 papers
Recent·Most cited
B. Fu, E. E. Brock, R. Andrews, J. C. Breiter, R. Tian, C. E. Toomey, J. Lachica, T. Lashley, M. Ryten, N. W. Wood, M. Vendruscolo, S. Gandhi, L. E. Weiss, J. S. Beckwith, S. F. Lee
RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.
bioRxiv · Dec 18, 2023Biophysics
G. Krainer, R. P. B. Jacquat, M. M. Schneider, T. J. Welsh, J. Fan, Q. A. E. Peter, E. A. Andrzejewska, G. Sneideriene, M. A. Czekalska, H. Ausserwoeger, L. Chai, W. E. Arter, K. L. Saar, T. W. Herling, T. M. Franzmann, , T. P. J. Knowles
This paper introduces smMDS, a method for digital sizing of proteins in solution, enabling sensitive and accurate measurement of protein sizes and interactions.
bioRxiv · Jul 12, 2023Biophysics
S. Daly, J. Ferreira Fernandes, E. Bruggeman, A. Handa, R. Peters, S. Benaissa, B. Zhang, J. S. Beckwith, E. W. Sanders, R. R. Sims, D. Klenerman, S. J. Davis, K. O'Holleran, S. F. Lee
This paper introduces SMLFM, a volumetric super-resolution microscopy technique that achieves higher speed and accuracy in detecting single molecules in live cells.
bioRxiv · May 2, 2023Biophysics
E. Bruggeman, O. Zhang, L.-M. Needham, M. Koerbel, S. Daly, M. Cheetham, R. Peters, T. Wu, A. S. Klymchenko, S. J. Davis, E. K. Paluch, D. Klenerman, M. D. Lew, K. O'Holleran, S. F. Lee
POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.
bioRxiv · Feb 7, 2023Biophysics
E. Sanders, A. R. Carr, E. Bruggeman, M. Koerbel, S. I. Benaissa, R. Donat, A. M. Santos, J. McColl, K. O'Holleran, D. Klenerman, S. J. Davis, S. F. Lee, A. Ponjavic
The paper introduces resPAINT, a method that enhances volumetric super-resolution microscopy by actively controlling probe emission, achieving up to 50-fold faster localisation rates without sacrificing contrast.
bioRxiv · Apr 14, 2022Biophysics
R. R. Sims, S. Abdul Rehman, M. O. Lenz, A. Clark, E. W. Sanders, A. Ponjavic, L. Muresan, S. F. Lee, K. O'Holleran
This paper introduces SMLFM, a 3D single molecule localization technique with 20 nm precision, and demonstrates its application to image fixed eukaryotic cell membranes below the diffraction limit.
bioRxiv · May 20, 2020 · PreprintBiophysics
A. Spark, A. Kitching, D. Esteban-Ferrer, A. Handa, A. R. Carr, L.-M. Needham, A. Ponjavic, M. Da Cunha Santos, J. McColl, C. Leterrier, S. J. Davis, R. Henriques, S. F. Lee
vLUME is a VR software for visualizing 3D SMLM data, enabling segmentation and quantification of fluorescence puncta with high fidelity.
bioRxiv · Jan 20, 2020Biophysics
L.-M. Needham, J. Weber, J. A. Varela, J. W. B. Fyfe, D. T. Do, C. K. Xu, B. Keenlyside, R. Cliffe, D. Klenerman, C. M. Dobson, C. A. Hunter, S. E. Bohndiek, T. N. Snaddon, S. F. Lee
This paper introduces ThX, a new fluorescent probe for detecting amyloid aggregates, showing improved brightness and binding affinity over ThT.
bioRxiv · Jul 24, 2019Biophysics
R. A. Fernandes, K. A. Ganzinger, J. Tzou, P. Jonsson, S. F. Lee, M. Palayret, A. M. Santos, A. A. Carr, A. Ponjavic, V. T. Chang, C. Macleod, B. C. Lagerholm, A. E. Lindsay, O. Dushek, A. Tilevik, , D. Klenerman
This paper develops a model showing T-cell receptors discriminate ligands based on dwell-time in phosphatase-depleted contacts constrained by cell topography, predicting signaling potency without multi-step proof-reading.
bioRxiv · Nov 19, 2018Immunology
J. H. Felce, S. L. Latty, R. G. Knox, S. R. Mattick, Y. Lui, S. F. Lee, D. Klenerman, S. J. Davis
The paper analyzes 71 GPCRs from a single cell to show that Rhodopsin-family receptors predominantly exist as monomers rather than dimers.
bioRxiv · Mar 2, 2017Biochemistry