Author

A. S. Klymchenko

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 5 Cell Biology· 3

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 7 Approach:experimental assay· 5 Research Focus:cell signaling & trafficking· 4 Organism:human· 4 Assay:imaging· 4 Assay:fluorescence / live-cell microscopy· 3 Approach:microscopy & imaging analysis· 3 Research Focus:spatial domain detection· 3

Frequent Co-Authors

E. Sezgin· 4 shared D. I. Danylchuk· 3 shared C. Eggeling· 2 shared F. Ragaller· 2 shared A. Abhinav· 1 shared A. Arora· 1 shared A. Dasgupta· 1 shared A. Hanyaloglou· 1 shared

Activity by Year

2017-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 14 mutual co-author links

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4 shared papersBiophysics

E. Sezgin

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Plasma membrane order maps functional diversity in immune cells

L. A. Andronico, C. O. Gurdap, A. Arora, F. Ragaller, P. A. Sandoz, Y. Jiang, S. Giatrellis, L. de Boer, V. Carannante, S. Iskrak, J. Mikes, M. Buggert, A. Osterborg, B. Onfelt, A. S. Klymchenko, , E. Sezgin

The paper identifies immune cell subsets with distinct functional behaviors based on plasma membrane order using biophysical profiling and transcriptome analysis.

bioRxiv · Jan 15, 2024Biophysics

POLCAM: Instant molecular orientation microscopy for the life sciences

E. Bruggeman, O. Zhang, L.-M. Needham, M. Koerbel, S. Daly, M. Cheetham, R. Peters, T. Wu, A. S. Klymchenko, S. J. Davis, E. K. Paluch, D. Klenerman, M. D. Lew, K. O'Holleran, S. F. Lee

POLCAM provides a fast, simplified method for single-molecule orientation microscopy using polarization cameras, enabling rapid molecular anisotropy analysis in biological samples.

bioRxiv · Feb 7, 2023Biophysics

Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties

F. Ragaller, L. Andronico, J. Sykora, W. Kulig, T. Rog, Y. B. Urem, M. Amaro, A. Abhinav, D. I. Danylchuk, A. S. Klymchenko, M. Hof, I. Vattulainen, E. Sezgin

This study investigates the sensitivity of three environment-sensitive probes to biophysical membrane properties using model systems and simulations.

bioRxiv · Jun 5, 2022Biophysics

Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway

T. Sokoya, J. Parolek, M. M. Foged, D. I. Danylchuk, M. Bozan, B. Sarkar, A. Hilderink, M. Philippi, L. D. Botto, P. A. Terhal, O. Maekitie, J. Piehler, Y. Kim, C. G. Burd, A. S. Klymchenko, , J. C. M. Holthuis

The paper shows pathogenic SMS2 variants disrupt lipid landscapes in the secretory pathway by trapping sphingomyelin in the ER and altering cholesterol and glycerophospholipid distributions.

bioRxiv · Apr 2, 2022Cell Biology

STED super-resolution imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes

P. Carravilla, A. Dasgupta, G. Zhurgenbayeva, D. I. Danylchuk, A. S. Klymchenko, E. Sezgin, C. Eggeling

This study demonstrates the use of NR4A, an exchangeable polarity-sensitive dye, for real-time 3D-STED imaging of membrane dynamics and biophysical properties in live cells.

bioRxiv · Jun 5, 2021Biophysics

Agonist binding affinity determines palmitoylation of the glucagon-like peptide-1 receptor and its functional interaction with plasma membrane nanodomains in pancreatic beta cells

T. Buenaventura, W. E. Laughlin, S. Bitsi, T. Burgoyne, Z. Lyu, A. I. Oqua, H. Norman, E. R. McGlone, A. S. Klymchenko, I. R. Correa, A. Walker, A. Inoue, A. Hanyaloglou, G. A. Rutter, S. R. Bloom, , A. Tomas

This study shows that agonist binding affinity affects GLP-1R palmitoylation, nanodomain clustering, and signaling in pancreatic beta cells, influencing insulin secretion.

bioRxiv · Dec 10, 2018Cell Biology

Studying the fate of tumor extracellular vesicles at high spatio-temporal resolution using the zebrafish embryo

V. Hyenne, S. Ghoroghi, M. Collot, S. Harlepp, J. Bauer, L. Mercier, I. Busnelli, O. Lefebvre, N. Fekonja, P. Machado, J. Bons, F. Delalande, A. I. Amor, S. G. Silva, F. J. Verweij, , J. G. Goetz

This study tracks tumor extracellular vesicles in zebrafish embryos using fluorescent probes, showing their uptake by endothelial cells and macrophages and their behavior in vivo.

bioRxiv · Jul 30, 2018Cell Biology

Polarity sensitive probes for super resolution STED microscopy

E. Sezgin, F. Schneider, V. Zilles, E. Garcia, D. Waithe, A. S. Klymchenko, C. Eggeling

This study evaluates polarity-sensitive dyes in super resolution STED microscopy to probe nanoscale membrane heterogeneity in cell-derived vesicles, live cells, and virus particles.

bioRxiv · Feb 9, 2017Biophysics