R. Sit

Ageing compromises mouse thymus function and remodels epithelial cell differentiation

This study investigates how ageing affects mouse thymus function, revealing that progenitor cell differentiation is disrupted, leading to impaired T cell selection and self-antigen representation.

Designing and implementing programmable depletion in sequencing libraries with DASHit

The paper introduces DASHit, a software tool that designs CRISPR-based depletion targets to reduce high-abundance sequences in sequencing libraries, demonstrating its effectiveness across three species.

Heterogeneity and targeted therapy-induced adaptations in lung cancer revealed by longitudinal single-cell RNA sequencing

This study uses longitudinal single-cell RNA sequencing to reveal therapy-induced adaptations in lung cancer, identifying cell state transitions and pathways associated with treatment response and resistance.

Investigating Transfusion-Related Sepsis using Culture-Independent Metagenomic Sequencing

This study uses metagenomic sequencing to identify pathogens in transfusion-related sepsis cases, revealing a novel Acinetobacter species and demonstrating the utility of culture-independent methods.

Serological and metagenomic interrogation of cerebrospinal fluid implicates enteroviruses in pediatric acute flaccid myelitis

This study identifies high levels of enterovirus antibodies in cerebrospinal fluid of children with acute flaccid myelitis compared to controls, suggesting a causal role for non-polio enteroviruses.

FLASH: A next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences

FLASH is a CRISPR-based diagnostic method that enhances detection of low-abundance antimicrobial resistance sequences in metagenomic data.