This study identifies and characterizes two new families of archaeal bactofilins, HalA and HalB, showing their roles in shaping cell morphology and curvature in Haloferax volcanii.

bioRxiv · Jan 22, 2024Microbiology

Identification and characterization of structural and regulatory cell-shape determinants in Haloferax volcanii

H. Schiller, J. Kouassi, Y. Hong, T. Rados, J. Kwak, A. DiLucido, D. Safer, A. Marchfelder, F. Pfeiffer, A. Bisson-Filho, S. Schulze, M. Pohlschroder

The paper identifies and characterizes proteins involved in cell-shape determination in Haloferax volcanii using proteomics, genetics, and live-cell imaging.

bioRxiv · Mar 5, 2023Microbiology

Advanced understanding of prokaryotic biofilm formation using a cost-effective and versatile multi-panel adhesion (mPAD) mount

S. Schulze, H. Schiller, J. Solomonic, O. Telhan, K. Costa, M. Pohlschroder

The paper introduces a 3D-printed mPAD mount for analyzing biofilm formation under varied conditions and shows phenazines are crucial for anoxic biofilm formation in Pseudomonas aeruginosa.

bioRxiv · Sep 9, 2021Microbiology

Glycoproteomics of Haloferax volcanii reveals an extensive glycoproteome and concurrence of different N-glycosylation pathways

S. Schulze, F. Pfeiffer, B. A. Garcia, M. Pohlschroder

The paper identifies an extensive N-glycoproteome in Haloferax volcanii and shows that different N-glycosylation pathways can modify the same sites under identical culture conditions.

bioRxiv · Jan 21, 2021Biochemistry

Haloferax volcanii immersed liquid biofilms develop independently of known biofilm machineries and exhibit rapid honeycomb pattern formation

H. Schiller, S. Schulze, Z. Mutan, C. de Vaulx, C. Runcie, J. Schwartz, T. Rados, A. W. Bisson Filho, M. Pohlschroder

mSphere · Dec 23, 2020Peer-reviewedMicrobiology

Improved growth and morphological plasticity of Haloferax volcanii

R. T. de Silva, M. F. Abdul Halim, D. A. Pittrich, H. J. Brown, M. Pohlschroder, I. G. Duggin

This study improves growth and morphological plasticity of Haloferax volcanii by supplementing trace elements, revealing the role of CetZ1 in shaping different cell forms.

bioRxiv · May 4, 2020Microbiology

LIPID ANCHORING OF ARCHAEOSORTASE SUBSTRATES AND MID-CELL GROWTH IN HALOARCHAEA

M. F. Abdul-Halim, S. Schulze, A. DiLucido, F. Pfeiffer, A. W. Bisson Filho, M. Pohlschroder

This study identifies a pathway in Haloferax volcanii where ArtA, PssA, and PssD co-localize at the mid-cell to facilitate lipid anchoring of surface proteins and mid-cell growth.

bioRxiv · Dec 4, 2019Cell Biology