A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus
S. Zeng, Y. Ju, M. S. Alam, Z. Lu, H. M. A. Hameed, L. Li, X. Tian, C. Fang, X. Fang, J. Ding, X. Wang, J. Hu, S. Wang, T. Zhang
This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.
bioRxiv · Jan 29, 2024Microbiology