X. Tian

Spatiotemporal transcriptomic profiling reveals the dynamic immunological landscape of alveolar echinococcosis

This study uses spatiotemporal transcriptomics to analyze immune dynamics in E. multilocularis infected mouse liver, identifying key immune cell roles and shifts in immune strategy during infection.

Protein S-glutathionylation confers cell resistance to ferroptosis

This study shows that protein S-glutathionylation protects cells from ferroptosis by maintaining ARF6 function and reducing labile iron pools.

Natural antibodies to polysaccharide capsules enable Kupffer cells to capture invading bacteria in the liver sinusoids

This study identifies natural antibodies that enable Kupffer cells to capture and kill encapsulated bacteria in the liver, offering a potential therapeutic approach for septic bacterial diseases.

Splenic red pulp macrophages eliminate the liver-resistant Streptococcus pneumoniae during bloodstream infection

The paper identifies that splenic red pulp macrophages, aided by natural antibodies and complement, clear liver-resistant Streptococcus pneumoniae from the bloodstream in mice.

MtrAB two-component system is crucial for the intrinsic resistance and virulence of Mycobacterium abscessus

This study shows that disrupting MtrA or MtrB in Mycobacterium abscessus increases antibiotic susceptibility and reduces virulence by affecting cell division and permeability.

Extracellular electron transfer drives efficient H2-independent methylotrophic methanogenesis by Methanomassiliicoccus, a seventh order methanogen

This study shows Methanomassiliicoccus uses extracellular electron transfer for efficient methylotrophic methanogenesis, enhancing methane production in coculture with electrogenic bacteria.

Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase

This paper develops a bioluminescent indicator for potassium ions using a modified luciferin and engineered luciferase, validated in cell lines, neurons, and live mice.

High-throughput Screening of Compounds against Autoluminescent Nonreplicating Mycobacterium tuberculosis under Diverse Conditions

This paper develops an autoluminescent model to assess anti-tuberculosis drugs against nonreplicating M. tuberculosis, showing real-time light output correlates with colony-forming units and reveals drug efficacy differences under anaerobic vs aerobic conditions.

Differentiating specific and non-specific protein-metabolite interactions using gradient open port probe electrospray ionization mass spectrometry

This paper introduces a method using gradient open port probe electrospray ionization mass spectrometry to distinguish specific from non-specific protein-metabolite interactions by analyzing charge state distributions under varying methanol concentrations.

Single-cell multiomics reveals a gene regulatory circuit promoting leukemia cell differentiation

This study uses single-cell multiomics to identify a gene regulatory circuit involving SPI1 and CEBPE that promotes differentiation in APL leukemia cells under ATRA treatment.

Establishment of an Inhalation Administration Noninvasive Murine Model for Rapidly Testing Drug Activity against Mycobacterium tuberculosis

This paper develops a non-invasive murine inhalation model using autoluminescent M. tuberculosis to rapidly assess drug efficacy against tuberculosis.

EmbB and EmbC Regulate the Sensitivity of Mycobacterium abscessus to Echinomycin

This study identifies EmbB and EmbC as regulators of Mycobacterium abscessus sensitivity to echinomycin, showing that EmbB mutations can partially compensate for EmbC loss and alter cell wall permeability.

Mutations in ClpC1 or ClpX subunit of the caseinolytic protease confer resistance to natural product ilamycins in mycobacteria

This study identifies two ilamycin analogs targeting mycobacterial Clp protease components and discovers mutations conferring resistance to these compounds.

A CRISPR-Nonhomologous End-Joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus

This paper introduces a CRISPR-NHEJ strategy for efficient gene disruption in Mycobacterium abscessus and shows that homologous recombination and single-strand annealing components are required for NHEJ repair.

Targeting transcription factors through an IMiD independent zinc finger domain

This study identifies a new IMiD-independent drug targeting SALL4's ZFC4 domain, leading to significant tumor growth inhibition in vivo.

Lithocholic acid phenocopies rejuvenating and life-extending effects of calorie restriction

This study identifies lithocholic acid as a metabolite that mimics calorie restriction's anti-aging effects in multiple species through AMPK activation.

Lithocholic acid targets TULP3 to activate sirtuins and AMPK to retard ageing

This study identifies LCA's mechanism of action through TULP3-sirtuin-v-ATPase-AMPK pathway to activate AMPK and extend lifespan in model organisms.

Linked regulation of genome integrity and senescence-associated inflammation by p53

This study identifies a mitochondria-regulated p53 circuit that links genomic instability and inflammation in senescent cells, showing p53 suppresses inflammation without affecting cell cycle arrest.

Local two-photon excitation reveals the mechanism of mitochondrial Ca2+ release

This study uses two-photon excitation to reveal that mitochondrial Ca2+ release is driven by ATP synthesis and the electron transport chain, not Ca2+ entry.

Regulation of Lung Immune Tone by the Gut-Lung Axis via Dietary Fiber, Gut Microbiota, and Short-Chain Fatty Acids

This study shows that dietary fiber and gut microbiota influence lung immune tone via short-chain fatty acids, reducing inflammation and lung injury in mice.

O-GlcNAcylation of Keratin 18 coordinates TCA cycle to promote cholangiocarcinoma progression

The paper shows O-GlcNAcylation of Keratin 18 coordinates TCA cycle enzymes to promote cholangiocarcinoma progression by stabilizing K18 and altering metabolite levels.

AMPK targets PDZD8 to trigger carbon source shift to glutamine

This paper identifies AMPK's role in shifting carbon utilization from glucose to glutamine via PDZD8 phosphorylation, enhancing glutaminolysis and extending lifespan in C. elegans and mice.

SALLB not targeted by IMiDs is important for maintenance of liver cancer cells

The paper identifies a compound that degrades both SALL4 isoforms, overcoming IMiD limitations in liver cancer treatment.

SeMOE allows for quantitative glycan perception and exhibits anti-cancer potentiality

SeMOE enables absolute glycan quantification and visualization through selenosugar-based metabolic oligosaccharide engineering, with demonstrated anti-cancer potential.

DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale

This study investigates DNA repair mechanisms in bowhead whales, showing their high efficiency and accuracy in repairing DNA damage, which may explain their long lifespan without cancer.

Increased hyaluronan by naked mole-rat HAS2 extends lifespan in mice

This study shows that overexpressing the naked mole-rat HAS2 gene in mice increases hyaluronan levels, reduces inflammation, and extends lifespan and healthspan.

Artificially cultivated duckweed: a high-efficiency starch producer

This paper reports a method to produce starch efficiently from duckweed under specific conditions and identifies mechanisms behind its high starch accumulation.

Duckweed evolution: from land back to water

This study investigates the reverse evolution of duckweed from land back to water, showing gradual degeneration in root structure, stomatal function, and lignocellulose content, along with gene pathway changes.

Expansion microscopy with trypsin digestion and tyramide signal amplification (TT-ExM) for protein and lipid staining

The paper introduces TT-ExM, an improved expansion microscopy technique that reduces protein loss and enhances fluorescence signals through trypsin digestion and tyramide signal amplification, enabling ultrafine-resolution imaging of cellular structures.

RelB represses miR-193a-5p expression to promote the phenotypic transformation of vascular smooth muscle cells in aortic aneurysm

This study identifies miR-193a-5p as a regulator of vascular smooth muscle cell proliferation and migration in aortic aneurysms, showing that RelB represses its expression.