Thinner is not always better: Optimising cryo lamellae for subtomogram averaging
M. W. Tuijtel, J. P. Kreysing, S. Welsch, G. Hummer, M. Beck, B. Turonova
This study benchmarks cryo-ET resolution based on lamella thickness and particle depth, showing no negative impact up to 180 nm and ion-induced damage extending 30 nm from surfaces.
bioRxiv · Jul 31, 2023Biophysics