M. Beck

Passage of the HIV capsid cracks the nuclear pore

This study investigates how HIV-1 capsids pass through nuclear pores, finding that capsids retain their structure and cause NPC scaffold rings to crack.

In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography

This study uses cryo-ET to visualize copia retrotransposon structure in Drosophila cells, revealing nuclear clustering and size selection during gametogenesis.

Nuclear pores safeguard the integrity of the nuclear envelope

This study shows that nuclear pore complexes dilate during cell differentiation and that Nup133 deficiency prevents this dilation, leading to nuclear envelope instability.

Effects of water nutrient concentrations on stream macroinvertebrate community stoichiometry: a large-scale study

This study examines how water phosphorus and nitrogen levels affect stream macroinvertebrate community stoichiometry using a large dataset from France.

Nuclear pores as conduits for fluid flow during osmotic stress

This study shows that Dictyostelium discoideum uses nuclear pores as conduits for fluid flow during osmotic stress, distinct from passive diffusion and active transport.

Thinner is not always better: Optimising cryo lamellae for subtomogram averaging

This study benchmarks cryo-ET resolution based on lamella thickness and particle depth, showing no negative impact up to 180 nm and ion-induced damage extending 30 nm from surfaces.

The killing of human gut commensal E. coli ED1a by tetracycline is associated with severe ribosome dysfunction

The paper examines how tetracycline affects ribosome function in two E. coli strains, showing different outcomes based on the antibiotic's bacteriostatic or bactericidal effect.

Translation dynamics in human cells visualized at high-resolution reveal cancer drug action

The paper visualizes ribosome dynamics in human cells at near-atomic resolution, revealing how a cancer drug alters translation.

miR-29 is an important driver of aging-related phenotypes

This study identifies miR-29 as a key driver of aging-related phenotypes by showing its upregulation in aging models and its ability to induce aging traits when overexpressed.

Bacterial cell widening alters periplasmic size and activates envelope stress responses

This study shows that changes in bacterial cell width affect periplasmic thickness and activate the Rcs stress response system through RcsF.

Deciphering the conformations and dynamics of FG-nucleoporins in situ

This study uses fluorescence microscopy and modeling to reveal the conformation and dynamics of FG-NUPs within the nuclear pore complex, showing good-solvent conditions facilitate transport.

Protein-Peptide Turnover Profiling reveals wiring of phosphorylation during protein maturation

The paper uses metabolic labeling and PTM-specific enrichment to reveal the temporal order of phosphorylation events during protein maturation, not protein stability.

A Modular Platform for Streamlining Automated Cryo-FIB Workflows

The paper introduces SerialFIB, an open-source software tool for automating cryo-FIB workflows, enabling customizable and efficient lamella preparation for cryo-electron tomography.

3D super-resolution fluorescence microscopy maps the variable molecular architecture of the Nuclear Pore Complex

The paper uses 3D super-resolution microscopy and computational methods to map the 3D structure of the Nuclear Pore Complex in human cells, revealing variable conformations of its subunits.

In cell architecture of the nuclear pore complex and snapshots of its turnover

The paper uses structural biology and yeast genetics to study NPC architecture and turnover in yeast cells, revealing new insights into mRNA export and autophagy mechanisms.

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

The paper shows HIV-1 capsids can enter the nucleus intact through nuclear pores, challenging the idea that uncoating must occur before nuclear import.

Nuclear pores constrict upon energy depletion

This study shows that nuclear pore complexes constrict when cells experience energy depletion, linking cellular energy status to NPC conformation.

An ESCRT-LEM domain inner nuclear membrane protein surveillance system is poised to directly monitor the integrity of the nuclear envelope barrier and nuclear transport system

The paper identifies a surveillance system in yeast that detects and seals nuclear envelope damage through ESCRT and inner nuclear membrane proteins.

Dead or Alive? Molecular life-dead distinction in human stool samples reveals significantly different composition of the microbial community

This study uses PMA treatment to distinguish live from dead microbes in human stool samples, showing that 100 M PMA effectively reduces signals from dead cells.

In situ architecture of the algal nuclear pore complex

This study reports the in situ architecture of the algal nuclear pore complex in Chlamydomonas reinhardtii and compares it to the human NPC, revealing structural variations between species.

Postmitotic Nuclear Pore Assembly Proceeds By Radial Dilation Of Small ER Membrane Openings

The paper shows that postmitotic nuclear pore assembly occurs through dilation of ER membrane openings, forming transport channels rapidly.