Metabolic selection of a homologous recombination mediated loss of glycosomal fumarate reductase in Trypanosoma brucei
M. wargnies, N. Plazolles, R. Schenk, O. Villafraz, J.-W. Dupuy, M. Biran, S. Bachmaier, H. Baudouin, C. Clayton, M. Boshart, F. Bringaud
The paper shows that a deletion event between FRDg and FRDm2 genes is selected in PEPCK knockout cells, leading to a non-functional chimeric gene that reduces growth, suggesting FRD's role in ROS production for parasite differentiation.
bioRxiv · Nov 29, 2020Microbiology